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transposon vector psam arac  (Addgene inc)


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    Addgene inc transposon vector psam arac
    a Schematic diagram of the screening experiments. The <t>transposon</t> mutant library of Aquitalea magnusonii H3rifR was inoculated to flasks with and without duckweed, and the DNA of mutants was collected from three fractions after 3 h or 7 d. Samples were destructively obtained from four flasks, and pooled before DNA extraction. b The abundance of mutant cells in the three fractions during the screening experiments. Error bars show standard deviation. c Detection frequency of genes in the three fractions after 3 h and 7 d. Log 10 gene-level detection frequencies were compared. Depleted and enriched genes were determined based on the effect size (fold change > 2) and statistical significance ( q < 0.05) among Plant and Control samples. Statistical test was performed by considering different mutants (insertion sites) of the same gene as replicates. d Comparison of the screening results of 3-h and 7-d experiments. Genes showed significant depletion or enrichment in the 3-h and 7-d experiments are denoted by crosses and squares, respectively.
    Transposon Vector Psam Arac, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transposon vector psam arac/product/Addgene inc
    Average 93 stars, based on 4 article reviews
    transposon vector psam arac - by Bioz Stars, 2026-02
    93/100 stars

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    1) Product Images from "Genome-wide identification of bacterial colonization and fitness determinants on the floating macrophyte, duckweed"

    Article Title: Genome-wide identification of bacterial colonization and fitness determinants on the floating macrophyte, duckweed

    Journal: Communications Biology

    doi: 10.1038/s42003-022-03014-7

    a Schematic diagram of the screening experiments. The transposon mutant library of Aquitalea magnusonii H3rifR was inoculated to flasks with and without duckweed, and the DNA of mutants was collected from three fractions after 3 h or 7 d. Samples were destructively obtained from four flasks, and pooled before DNA extraction. b The abundance of mutant cells in the three fractions during the screening experiments. Error bars show standard deviation. c Detection frequency of genes in the three fractions after 3 h and 7 d. Log 10 gene-level detection frequencies were compared. Depleted and enriched genes were determined based on the effect size (fold change > 2) and statistical significance ( q < 0.05) among Plant and Control samples. Statistical test was performed by considering different mutants (insertion sites) of the same gene as replicates. d Comparison of the screening results of 3-h and 7-d experiments. Genes showed significant depletion or enrichment in the 3-h and 7-d experiments are denoted by crosses and squares, respectively.
    Figure Legend Snippet: a Schematic diagram of the screening experiments. The transposon mutant library of Aquitalea magnusonii H3rifR was inoculated to flasks with and without duckweed, and the DNA of mutants was collected from three fractions after 3 h or 7 d. Samples were destructively obtained from four flasks, and pooled before DNA extraction. b The abundance of mutant cells in the three fractions during the screening experiments. Error bars show standard deviation. c Detection frequency of genes in the three fractions after 3 h and 7 d. Log 10 gene-level detection frequencies were compared. Depleted and enriched genes were determined based on the effect size (fold change > 2) and statistical significance ( q < 0.05) among Plant and Control samples. Statistical test was performed by considering different mutants (insertion sites) of the same gene as replicates. d Comparison of the screening results of 3-h and 7-d experiments. Genes showed significant depletion or enrichment in the 3-h and 7-d experiments are denoted by crosses and squares, respectively.

    Techniques Used: Mutagenesis, DNA Extraction, Standard Deviation, Control, Comparison



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    transposon vector psam arac  (Addgene inc)
    93
    Addgene inc transposon vector psam arac
    a Schematic diagram of the screening experiments. The <t>transposon</t> mutant library of Aquitalea magnusonii H3rifR was inoculated to flasks with and without duckweed, and the DNA of mutants was collected from three fractions after 3 h or 7 d. Samples were destructively obtained from four flasks, and pooled before DNA extraction. b The abundance of mutant cells in the three fractions during the screening experiments. Error bars show standard deviation. c Detection frequency of genes in the three fractions after 3 h and 7 d. Log 10 gene-level detection frequencies were compared. Depleted and enriched genes were determined based on the effect size (fold change > 2) and statistical significance ( q < 0.05) among Plant and Control samples. Statistical test was performed by considering different mutants (insertion sites) of the same gene as replicates. d Comparison of the screening results of 3-h and 7-d experiments. Genes showed significant depletion or enrichment in the 3-h and 7-d experiments are denoted by crosses and squares, respectively.
    Transposon Vector Psam Arac, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transposon vector psam arac/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    transposon vector psam arac - by Bioz Stars, 2026-02
    93/100 stars
    		  Buy from Supplier

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    a Schematic diagram of the screening experiments. The transposon mutant library of Aquitalea magnusonii H3rifR was inoculated to flasks with and without duckweed, and the DNA of mutants was collected from three fractions after 3 h or 7 d. Samples were destructively obtained from four flasks, and pooled before DNA extraction. b The abundance of mutant cells in the three fractions during the screening experiments. Error bars show standard deviation. c Detection frequency of genes in the three fractions after 3 h and 7 d. Log 10 gene-level detection frequencies were compared. Depleted and enriched genes were determined based on the effect size (fold change > 2) and statistical significance ( q < 0.05) among Plant and Control samples. Statistical test was performed by considering different mutants (insertion sites) of the same gene as replicates. d Comparison of the screening results of 3-h and 7-d experiments. Genes showed significant depletion or enrichment in the 3-h and 7-d experiments are denoted by crosses and squares, respectively.

    Journal: Communications Biology

    Article Title: Genome-wide identification of bacterial colonization and fitness determinants on the floating macrophyte, duckweed

    doi: 10.1038/s42003-022-03014-7

    Figure Lengend Snippet: a Schematic diagram of the screening experiments. The transposon mutant library of Aquitalea magnusonii H3rifR was inoculated to flasks with and without duckweed, and the DNA of mutants was collected from three fractions after 3 h or 7 d. Samples were destructively obtained from four flasks, and pooled before DNA extraction. b The abundance of mutant cells in the three fractions during the screening experiments. Error bars show standard deviation. c Detection frequency of genes in the three fractions after 3 h and 7 d. Log 10 gene-level detection frequencies were compared. Depleted and enriched genes were determined based on the effect size (fold change > 2) and statistical significance ( q < 0.05) among Plant and Control samples. Statistical test was performed by considering different mutants (insertion sites) of the same gene as replicates. d Comparison of the screening results of 3-h and 7-d experiments. Genes showed significant depletion or enrichment in the 3-h and 7-d experiments are denoted by crosses and squares, respectively.

    Article Snippet: The mutant library of H3rifR was created with transposon vector pSAM_AraC (Addgene plasmid #91569).

    Techniques: Mutagenesis, DNA Extraction, Standard Deviation, Control, Comparison